Identification and Genetic Analysis of Resistance of a Rice Germplasm 911A② to Rice Gall Midges

doi:10.3969/j.issn.10017216.2012.04.018

Abstract

Abstract: The resistance of a rice germplasm 911A② to the rice gall midge(GM) was identified and genetically analyzed. 911A② was used as male parent to hybridize, test cross with Jinggui, TN1, W1263（Gm1）, IET2911（Gm2）, BG4041（gm3）, OB677（Gm4）, ARC5984（Gm5）and Duokang 1（Gm6）, and their F1 selfpollinated， respectively. The resistance of all parental lines and the populations of F1, BC1F1, F2 to the rice gall midge were identified. The resullts showed that 911A② and all F1 resist to rice gall midge biotype Ⅳ of China. The segregation ratio of resistant plants to sensitive ones to rice gall midge biotype Ⅳ of China of BC1F1 and F2 accorded with 1(resistant)∶3(sensitive) and 9(resistant)∶7(sensitive) rule by χ2 test, suggesting that the resistance of 911A② to rice gall midge biotype Ⅳ of China was controlled by two dominant genes, which was nonallelic to the known resistant genes Gm1, Gm2, gm3, Gm4, Gm5and Gm6, as well as the other five resistant genes Gm7，Gm8，Gm9，Gm10, Gm11（t）. It suggested that the two genes are new rice gall midge resistance genes.

Key words: rice, rice gall midge, resistance test； resistant gene

摘要： 以抗虫品种资源911A②为父本，分别与粳桂、TN1、W1263（Gm1）、 IET2911（Gm2）、BG4041（gm3）、OB677（Gm4）、ARC5984（Gm5）和多抗1号（Gm6）（母本）进行杂交、测交、自交，获得F1、BC1F1、F2群体，对亲本和各个群体进行稻瘿蚊的抗性鉴定，研究911A②的抗性基因和遗传规律。结果表明，911A②及其与其他品种杂交获得的F1都抗中国稻瘿蚊生物型Ⅳ型，杂交获得的BC1F1和F2对中国稻瘿蚊生物型Ⅳ型的抗虫和感虫植株分离比经χ2测验分别符合1∶3和9∶7，说明911A②对中国稻瘿蚊生物型Ⅳ型的抗性受2对显性基因控制。911A②所含的2对抗性基因与已知的6个抗性基因Gm1、Gm2、gm3、Gm4、Gm5和Gm6不等位，与其他5个抗性基因Gm7、Gm8、Gm9、Gm10、Gm11(t)也不等位。该抗性基因可能是2对新的抗性基因。

关键词: 水稻, 稻瘿蚊, 抗性鉴定, 抗性基因

CLC Number:

LU Jiashi1,2, HE Longfei1,*, XU Jing1, XU Chunyan1, LI Chuangzhen1, WEI Sumei3. Identification and Genetic Analysis of Resistance of a Rice Germplasm 911A② to Rice Gall Midges[J]. Chinese Journal of Rice Science, 2012, 26(4): 506-510.

卢家仕1,2,何龙飞1,* ,徐晶1,许春燕1,李创珍1 ,韦素美3. 水稻品种资源911A②对稻瘿蚊的抗性鉴定及其遗传分析[J]. 中国水稻科学, 2012, 26(4): 506-510.

References

\[1\]刘名镇, 徐小红, 鄢祖林, 等. 抗稻瘿蚊种质资源的筛选与利用. 中国种业, 2003(4): 40.

\[2\]谭玉娟, 潘英, 莫禹诗,等. 抗稻瘿蚊水稻品种的筛选鉴定. 广东农业科学, 1982(6): 3233.

\[3\]董海, 李创珍, 金孟肖. 抗稻瘿蚊水稻品种的筛选鉴定研究. 昆虫知识, 1989（5）: 14.

\[4\]韦素美,黄凤宽,罗善昱,等.广西水稻新品种(系)及稻种资源对稻瘿蚊的抗性分析. 广西农业科学.，2003（3）: 4748.

\[5\]韦素美,黄凤宽,师翱翔,等.外引水稻品种抗稻瘿蚊鉴定.广西农业科学,1994(1): 2629.

\[6\]韦素美,黄凤宽,罗善昱等.国际水稻遗传评价试验网材料对稻褐飞虱和稻瘿蚊的抗性鉴定.植物保护,2004,30(3):2426.

\[7\]黎国涛,谭玉娟,潘英.稻瘿蚊生物型的研究.植物保护学报, 1983, 10(3): 147152.

\[8\]韦素美,黄凤宽,罗善昱,等.广西稻瘿蚊生物型测定及抗源评价利用.广西农业生物科学,2003,22(1):1015.

\[9\]Biradar S K, Sundaram R M,Thirumurugan T, et al. Identification of flanking SSR markers for a major rice gall midge resistance gene Gm1 and their validation. Theor Appl Genet (2004)109: 14681473．

\[10\]Mohan M, Nair S, Bentur J S, et al. RFLP and RAPD mapping of the rice Gm2 gene that confers resistance to biotype 1 of gall midge (Orseolia oryzae). Theor Appl Genet,1994, 87:782788．

\[11\]Khush G S, Brar D S, Hardy B. Advances in Rice Genetics. Los Banos, the philippines: International Rice Research Institute, 2003: 5154．

\[13\]John Milton Lima, Abhishek Dass, Sahu S C, et al. A RAPD marker identified a susceptible specific locus for gall midge resistance gene in rice cultivar ARC5984. Crop Prot, 2007, 26:14311435．

\[14\]张扬, 谭玉娟, 黄炳超, 等. 多抗1号抗稻瘿蚊遗传研究. 植物保护学报, 2000, 27(2): 136140．

\[15\]Sardesai N, Kumar A, Rajyashri K R, et al. Identification and mapping of an AFLP marker linked to Gm 7, a gall midge resistance gene and its conversion to a SCAR marker for its utility in marker aided selection in rice. Theor Appl Genet, 2002, 105: 691698．

\[16\]Jain A, Ariyadasa R, Kumar A, et al. Tagging and mapping of a rice gall midge resistance gene, Gm 8, and development of SCARs for use in markeraided selection and gene pyramiding. Theor Appl Genet, 2004, 109: 13771384．

\[17\]Shrivastava M N, Kumar A, Bhandarkar S, et al. A new gene for resistance in rice to Asian rice gall midge ( Orseolia oryzae Wood Mason) biotype 1 population at Raipur. India Euphyt, 2003, 130: 143145．

\[18\]Kumar A, Jain A, Sahu R K, et al. Genetic analysis of resistance genes for the rice gall midge in two rice genotypes. Crop Sci, 2005, 45(4): 16311635．

\[19\]Himabindu K, Suneetha K, Sama V S A K, et al. A new rice gall midge resistance gene in the breeding line CR57  MR1523, mapping with flanking markers and development of NILs. Euphytica, 2010，174: 179187．

\[20\]Katiyar S, Verulkar S, Chandel G, et al. Genetic analysis and pyramiding of two gall midge resistance genes (Gm2 and Gm6t)in rice(Oryza sativa L.). Euphytica, 2001, 122：327334．

\[21\]黄炳超, 张扬, 肖汉祥. 我国抗稻瘿蚊育种研究的新进展. 作物研究, 2004, (4): 201203．

\[22\]卢家仕, 何龙飞, 徐晶, 等. 稻瘿蚊饲养试验研究. 安徽农业科学, 2011, 39(28）: 1729117292, 17303．

\[23\]Heinrichs E A, Medrano F G, Rapsas H R. Genetic evaluation for insect resistance in rice Los Banos, Philippines: International Rice Research Institute,1985, 6567．

\[24\]郭辉, 冯锐, 秦学毅, 等. 水稻抗稻瘿蚊基因的研究与利用现状. 杂交水稻, 2010, 25(1): 48．

\[25\]Alam S N, Cohen M B. Durability of brown plant hopper, N ilaparvata lugens, resistance in rice variety IR64 in greenhouse selection studies. Entomol Exp Appl, 1998, 89: 7178．

\[26\]黄凤宽, 韦素美, 梁广文, 等. 水稻品种 RP1976218262422 对褐飞虱和稻瘿蚊的抗性评价及其遗传分析. 中国水稻科学, 2006, 20(1) :113115．

\[27\]Rawat N, Sinha D K, Rajendrakumar P, et al ．Role of pathogenesisrelated genes in ricegall midge interactions. Current Sci, 2010, 99(10): 13611368．

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